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Merck KGaA
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GeneTex
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Image Search Results
Journal: bioRxiv
Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K + channels
doi: 10.1101/2021.01.21.427626
Figure Lengend Snippet: (A) Immunostaining of mouse cortex using antibodies against AnkR (red) and β 1 spectrin (green). Scalebar, 20 µm. (B) Immunoblot of β 1 spectrin, AnkR, and Caspr immunoprecipitation reactions using antibodies against AnkR and β 1 spectrin. IP, immunoprecipitation; IB, immunoblot. (C) Immunoblot of control and AnkR-deficient mouse brains using antibodies against β 1 spectrin, AnkR, and neurofilament-M (NFM). Quantification of the β 1 spectrin immunoblots normalized to NFM. Error bars indicate mean ± SEM. N=6. (D) Top AnkR-interacting candidates. Circle size corresponds to the mean PSM from IP mass spectrometry. Concentric circles radiating from Ank1 correspond to % reduction in PSMs from AnkR knockout mouse compared to control. Identified proteins are organized according to their putative functions. ( E-H ) Immunostaining of mouse cortex using antibodies against AnkR (red) and TnR (green, E ), Bcan (green, F ), Acan (green, G ), and Vcan (green, H ). Scalebars, 20 μm. (I) Immunostaining of mouse cortex and hippocampus using antibodies against AnkR (red) and fluorescent-labeled WFA (green). Scalebars, 250 µm (top) and 20 µm (bottom). (J) Immunostaining of control (left) and AnkR-deficient mouse brain (right) using antibodies against AnkR (red) and NrCAM (green). Scalebars, 50 µm. (K) Immunoblot of NrCAM, AnkR, and Caspr immunoprecipitation reactions using antibodies against AnkR (top) and NrCAM (bottom). (L) Immunoblot of PlexinA4, AnkR, and Caspr immunoprecipitation reactions using antibodies against AnkR (top). Immunoblot of Nrp1, AnkR, and Caspr immunoprecipitation reactions using antibodies against AnkR (bottom).
Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (UC Davis/NIH NeuroMab Facility Cat# 75-380, RRID:AB_2491109), β 1 spectrin (UC Davis/NIH NeuroMab Facility Cat# 73-374, RRID:AB_2315814), AnkG (UC Davis/NIH NeuroMab Facility Cat# 73-146, RRID:AB_10697718), parvalbumin (UC Davis/NIH NeuroMab Facility Cat# 73-455, RRID:AB_2629420), actin (Millipore Cat# MAB1501, RRID:AB_2223041), tenascinR (R and D Systems Cat# MAB1624, RRID:AB_2207001), aggrecan (Millipore Cat# AB1031, RRID:AB_90460), brevican (UC Davis/NIH NeuroMab Facility Cat# 75-294, RRID:AB_2315824), NrCAM (R and D Systems Cat# MAB2034, RRID:AB_2267411), Kv3.1b (UC Davis/NIH NeuroMab Facility Cat# N16B/8, RRID:AB_2750730 and Thermo Fisher Cat# MA5-27684, RRID:AB_2735238), Kv3.3 (Antibodies-Online Cat# ABIN572016, RRID:AB_10782137), Kv7.2 (James Trimmer, University of California at Davis Cat# N26A/23, RRID:AB_2750761), Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR( ) (RRID:AB_2833096), Ank1 (Thermo Fisher Scientific Cat# PA5-63372, RRID:AB_2638015), neurofilament M (Millipore Cat# AB1987, RRID:AB_91201), parvalbumin (Novus Cat# NB120-11427, RRID:AB_791498), versican (Millipore Cat# AB1032, RRID:AB_11213831), PlexinA4 (Abcam Cat# ab39350, RRID:AB_944890), and
Techniques: Immunostaining, Western Blot, Immunoprecipitation, Mass Spectrometry, Knock-Out, Labeling
Journal: bioRxiv
Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K + channels
doi: 10.1101/2021.01.21.427626
Figure Lengend Snippet: ( A, B ) Immunostaining of cortex ( A ) and hippocampus ( B ) in control and AnkR-deficient mouse brain using antibodies against AnkR (red), β 1 spectrin (green), and the nuclear marker Hoechst. Scale bars, 50 µm. ( C ) Proteomics strategy to identify AnkR-interacting proteins. PSM, peptide spectral match. ( D, E ) Immunostaining of cortex ( D ) and hippocampus ( E ) using antibodies against AnkR (red) and NrCAM (green). Arrowheads in ( E ) indicate AnkR + /NrCAM + neurons. Scalebars, 20 µm ( D ), 50 μm ( E ). ( F ) Immunofluorescence of hippocampus using fluorescent WFA (red) and antibodies against NrCAM (green). Arrowheads indicate WFA + neurons. Scalebar, 25 µm. ( G, H ) Immunostaining of caudoputamen in control and AnkR-deficient mouse brain using fluorescent WFA (red) and antibodies against NrCAM (green). Arrowheads indicate WFA + neurons. Scalebars, 50 µm. ( I ) Staining of deep cerebellar nuclei using antibodies against Nrp1 (green) and AnkR (red). Scalebar, 100 µm.
Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (UC Davis/NIH NeuroMab Facility Cat# 75-380, RRID:AB_2491109), β 1 spectrin (UC Davis/NIH NeuroMab Facility Cat# 73-374, RRID:AB_2315814), AnkG (UC Davis/NIH NeuroMab Facility Cat# 73-146, RRID:AB_10697718), parvalbumin (UC Davis/NIH NeuroMab Facility Cat# 73-455, RRID:AB_2629420), actin (Millipore Cat# MAB1501, RRID:AB_2223041), tenascinR (R and D Systems Cat# MAB1624, RRID:AB_2207001), aggrecan (Millipore Cat# AB1031, RRID:AB_90460), brevican (UC Davis/NIH NeuroMab Facility Cat# 75-294, RRID:AB_2315824), NrCAM (R and D Systems Cat# MAB2034, RRID:AB_2267411), Kv3.1b (UC Davis/NIH NeuroMab Facility Cat# N16B/8, RRID:AB_2750730 and Thermo Fisher Cat# MA5-27684, RRID:AB_2735238), Kv3.3 (Antibodies-Online Cat# ABIN572016, RRID:AB_10782137), Kv7.2 (James Trimmer, University of California at Davis Cat# N26A/23, RRID:AB_2750761), Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR( ) (RRID:AB_2833096), Ank1 (Thermo Fisher Scientific Cat# PA5-63372, RRID:AB_2638015), neurofilament M (Millipore Cat# AB1987, RRID:AB_91201), parvalbumin (Novus Cat# NB120-11427, RRID:AB_791498), versican (Millipore Cat# AB1032, RRID:AB_11213831), PlexinA4 (Abcam Cat# ab39350, RRID:AB_944890), and
Techniques: Immunostaining, Marker, Immunofluorescence, Staining