mab1624 anti versican Search Results


mab1624 anti versican  (R&D Systems)
93
R&D Systems mab1624 anti versican
Mab1624 Anti Versican, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
mab1624 anti versican - by Bioz Stars, 2026-03
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kv3 1b  (Alomone Labs)
93
Alomone Labs kv3 1b
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Kv3 1b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv3 1b/product/Alomone Labs
Average 93 stars, based on 1 article reviews
kv3 1b - by Bioz Stars, 2026-03
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r d systems mab1624 anti versican  (R&D Systems)
92
R&D Systems r d systems mab1624 anti versican
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
R D Systems Mab1624 Anti Versican, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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anti-nav 1.6  (Millipore)
90
Millipore anti-nav 1.6
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Anti Nav 1.6, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-bral1  (Abnova)
90
Abnova anti-bral1
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Anti Bral1, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti-bral1 - by Bioz Stars, 2026-03
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rat  (R&D Systems)
92
R&D Systems rat
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Rat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat/product/R&D Systems
Average 92 stars, based on 1 article reviews
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af4009 anti caspr  (R&D Systems)
93
R&D Systems af4009 anti caspr
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Af4009 Anti Caspr, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioplan 2 microscope  (Carl Zeiss)
90
Carl Zeiss axioplan 2 microscope
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Axioplan 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab5210 anti tenascin r human human  (R&D Systems)
92
R&D Systems mab5210 anti tenascin r human human
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Mab5210 Anti Tenascin R Human Human, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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fluorsave reagent  (Merck KGaA)
90
Merck KGaA fluorsave reagent
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Fluorsave Reagent, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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r d systems af3865 anti tenascin r rat wb mouse  (R&D Systems)
93
R&D Systems r d systems af3865 anti tenascin r rat wb mouse
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
R D Systems Af3865 Anti Tenascin R Rat Wb Mouse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mosaix extension in the axiovision software  (Carl Zeiss)
90
Carl Zeiss mosaix extension in the axiovision software
( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and <t>Kv3.1b</t> (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
Mosaix Extension In The Axiovision Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and Kv3.1b (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.

Journal: bioRxiv

Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K + channels

doi: 10.1101/2021.01.21.427626

Figure Lengend Snippet: ( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and Kv3.1b (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.

Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (UC Davis/NIH NeuroMab Facility Cat# 75-380, RRID:AB_2491109), β 1 spectrin (UC Davis/NIH NeuroMab Facility Cat# 73-374, RRID:AB_2315814), AnkG (UC Davis/NIH NeuroMab Facility Cat# 73-146, RRID:AB_10697718), parvalbumin (UC Davis/NIH NeuroMab Facility Cat# 73-455, RRID:AB_2629420), actin (Millipore Cat# MAB1501, RRID:AB_2223041), tenascinR (R and D Systems Cat# MAB1624, RRID:AB_2207001), aggrecan (Millipore Cat# AB1031, RRID:AB_90460), brevican (UC Davis/NIH NeuroMab Facility Cat# 75-294, RRID:AB_2315824), NrCAM (R and D Systems Cat# MAB2034, RRID:AB_2267411), Kv3.1b (UC Davis/NIH NeuroMab Facility Cat# N16B/8, RRID:AB_2750730 and Thermo Fisher Cat# MA5-27684, RRID:AB_2735238), Kv3.3 (Antibodies-Online Cat# ABIN572016, RRID:AB_10782137), Kv7.2 (James Trimmer, University of California at Davis Cat# N26A/23, RRID:AB_2750761), Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR( ) (RRID:AB_2833096), Ank1 (Thermo Fisher Scientific Cat# PA5-63372, RRID:AB_2638015), neurofilament M (Millipore Cat# AB1987, RRID:AB_91201), parvalbumin (Novus Cat# NB120-11427, RRID:AB_791498), versican (Millipore Cat# AB1032, RRID:AB_11213831), PlexinA4 (Abcam Cat# ab39350, RRID:AB_944890), and neuropilin-1 (GeneTex Cat# GTX16786, RRID:AB_422398), Kv3.1b (Alomone Labs Cat# APC-014, RRID:AB_2040166), Kv3.3 (Alomone Labs Cat# APC-102, RRID:AB_2040170), GFP (Thermo Fisher Scientific, Cat# A-11122, RRID: AB_221569); and chicken polyclonal antibody against Neurofascin (R and D Systems Cat# AF3235, RRID:AB_10890736).

Techniques: Immunostaining, Immunofluorescence, Western Blot, Expressing, Binding Assay, Immunoprecipitation, Labeling

(A) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. (B) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (C) Quantification of Kv3.1b protein normalized to NFM. (D) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. (E) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. (F) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. (G) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind.

Journal: bioRxiv

Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K + channels

doi: 10.1101/2021.01.21.427626

Figure Lengend Snippet: (A) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. (B) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (C) Quantification of Kv3.1b protein normalized to NFM. (D) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. (E) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. (F) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. (G) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind.

Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (UC Davis/NIH NeuroMab Facility Cat# 75-380, RRID:AB_2491109), β 1 spectrin (UC Davis/NIH NeuroMab Facility Cat# 73-374, RRID:AB_2315814), AnkG (UC Davis/NIH NeuroMab Facility Cat# 73-146, RRID:AB_10697718), parvalbumin (UC Davis/NIH NeuroMab Facility Cat# 73-455, RRID:AB_2629420), actin (Millipore Cat# MAB1501, RRID:AB_2223041), tenascinR (R and D Systems Cat# MAB1624, RRID:AB_2207001), aggrecan (Millipore Cat# AB1031, RRID:AB_90460), brevican (UC Davis/NIH NeuroMab Facility Cat# 75-294, RRID:AB_2315824), NrCAM (R and D Systems Cat# MAB2034, RRID:AB_2267411), Kv3.1b (UC Davis/NIH NeuroMab Facility Cat# N16B/8, RRID:AB_2750730 and Thermo Fisher Cat# MA5-27684, RRID:AB_2735238), Kv3.3 (Antibodies-Online Cat# ABIN572016, RRID:AB_10782137), Kv7.2 (James Trimmer, University of California at Davis Cat# N26A/23, RRID:AB_2750761), Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR( ) (RRID:AB_2833096), Ank1 (Thermo Fisher Scientific Cat# PA5-63372, RRID:AB_2638015), neurofilament M (Millipore Cat# AB1987, RRID:AB_91201), parvalbumin (Novus Cat# NB120-11427, RRID:AB_791498), versican (Millipore Cat# AB1032, RRID:AB_11213831), PlexinA4 (Abcam Cat# ab39350, RRID:AB_944890), and neuropilin-1 (GeneTex Cat# GTX16786, RRID:AB_422398), Kv3.1b (Alomone Labs Cat# APC-014, RRID:AB_2040166), Kv3.3 (Alomone Labs Cat# APC-102, RRID:AB_2040170), GFP (Thermo Fisher Scientific, Cat# A-11122, RRID: AB_221569); and chicken polyclonal antibody against Neurofascin (R and D Systems Cat# AF3235, RRID:AB_10890736).

Techniques: Immunolabeling, Western Blot, Immunostaining

AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β 1- α 2 spectrin-based cytoskeleton. Loss of AnkR results in 1) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; 2) molecular changes including reduced β 1 spectrin, PNN-associated NrCAM, and Kv3.1b; 3) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and 4) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.

Journal: bioRxiv

Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K + channels

doi: 10.1101/2021.01.21.427626

Figure Lengend Snippet: AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β 1- α 2 spectrin-based cytoskeleton. Loss of AnkR results in 1) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; 2) molecular changes including reduced β 1 spectrin, PNN-associated NrCAM, and Kv3.1b; 3) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and 4) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.

Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (UC Davis/NIH NeuroMab Facility Cat# 75-380, RRID:AB_2491109), β 1 spectrin (UC Davis/NIH NeuroMab Facility Cat# 73-374, RRID:AB_2315814), AnkG (UC Davis/NIH NeuroMab Facility Cat# 73-146, RRID:AB_10697718), parvalbumin (UC Davis/NIH NeuroMab Facility Cat# 73-455, RRID:AB_2629420), actin (Millipore Cat# MAB1501, RRID:AB_2223041), tenascinR (R and D Systems Cat# MAB1624, RRID:AB_2207001), aggrecan (Millipore Cat# AB1031, RRID:AB_90460), brevican (UC Davis/NIH NeuroMab Facility Cat# 75-294, RRID:AB_2315824), NrCAM (R and D Systems Cat# MAB2034, RRID:AB_2267411), Kv3.1b (UC Davis/NIH NeuroMab Facility Cat# N16B/8, RRID:AB_2750730 and Thermo Fisher Cat# MA5-27684, RRID:AB_2735238), Kv3.3 (Antibodies-Online Cat# ABIN572016, RRID:AB_10782137), Kv7.2 (James Trimmer, University of California at Davis Cat# N26A/23, RRID:AB_2750761), Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR( ) (RRID:AB_2833096), Ank1 (Thermo Fisher Scientific Cat# PA5-63372, RRID:AB_2638015), neurofilament M (Millipore Cat# AB1987, RRID:AB_91201), parvalbumin (Novus Cat# NB120-11427, RRID:AB_791498), versican (Millipore Cat# AB1032, RRID:AB_11213831), PlexinA4 (Abcam Cat# ab39350, RRID:AB_944890), and neuropilin-1 (GeneTex Cat# GTX16786, RRID:AB_422398), Kv3.1b (Alomone Labs Cat# APC-014, RRID:AB_2040166), Kv3.3 (Alomone Labs Cat# APC-102, RRID:AB_2040170), GFP (Thermo Fisher Scientific, Cat# A-11122, RRID: AB_221569); and chicken polyclonal antibody against Neurofascin (R and D Systems Cat# AF3235, RRID:AB_10890736).

Techniques: Scaffolding, Injection